Quality system
ISO 9001:2015, certificate 04925Q01818R1S, valid to 19 December 2028. Scope: research, manufacture and sale of liquid chromatography packing and columns.
Base materials
Three U.S. FDA Type II Drug Master Files filed for the silica base materials: DMF 041399, 041400 and 041401.
Batch release
Each column is tested before shipping. The Certificate of Analysis reports dimensions, particle and pore size, carbon load, plate count, asymmetry, retention, back-pressure and batch number.
MicroPulite XP
Hybrid silica, 130 Å. The widest pH range (up to 1–12) for high- and low-pH methods, UHPLC and HPLC.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| XP tC18 | L1 | 1.7, 2.5, 3, 3.5, 5, 7 | 1–12 | 18% | Yes | Yes | 80 / 60 °C | 130 | 185 | Hybrid silica, C18, trifunctional | XBridge C18 |
| XP tC8 | L7 | 1.7, 2.5, 3, 3.5, 5 | 1–12 | 13% | Yes | Yes | 60 °C | 130 | 185 | Hybrid silica, C8, trifunctional | XBridge C8 |
| XP tC4 | L26 | 1.7, 2.5, 3, 3.5, 5 | 1–10 | 9.5% | Yes | Yes | 80 / 50 °C | 130 | 185 | Hybrid silica, C4, trifunctional | — |
| XP RP18 | L1 | 1.7, 2.5, 3, 3.5, 5, 10 | 1–12 | 18% | Yes | Yes | 50 / 45 °C | 130 | 185 | Hybrid silica, polar-embedded C18 | — |
| XP RP18 Plus | L1 | 1.7, 2.5, 3, 3.5, 5 | 2–12 | 18% | Yes | Yes | 50 / 45 °C | 130 | 185 | Hybrid silica, carbamate C18 | XBridge Shield RP18 |
| XP T3 | L1 | 3, 3.5, 5 | 1–12 | 14% | Yes | Yes | 80 / 60 °C | 130 | 185 | Hybrid silica, low-density C18 | — |
| XP Phenyl-Hexyl | L11 | 1.7, 2.5, 3, 3.5, 5 | 1–12 | 15% | Yes | No | 80 / 60 °C | 130 | 185 | Hybrid silica, phenyl-hexyl | XBridge Phenyl |
| XP Amide | L68 | 1.7, 2.5, 3, 3.5, 5, 10 | 2–11 | 12% | Yes | No | 80 °C | 130 | 185 | Hybrid silica, amide | XBridge Amide |
| XP HILIC | L3 | 1.7, 2.5, 3, 3.5, 5 | 1–9 | — | No | No | 45 °C | 130 | 185 | Hybrid silica, bare | XBridge HILIC |
| XP NH2 | L1 * | 3, 3.5, 5 | 1–9 | 9% | No | No | 45 °C | 130 | 185 | Hybrid silica, amino | — |
| XP tPFP | L43 | 1.7, 2.5, 3, 3.5, 5 | 1–8 | 7% | No | No | 45 °C | 130 | 185 | Hybrid silica, pentafluorophenyl | — |
| XP tC18/PFP | L43 | 1.7, 2.5, 3, 3.5, 5 | 1–12 | 14.5% | No | No | 45 °C | 130 | 185 | Hybrid silica, mixed-mode C18/PFP | — |
| XP tC18-AX | L78 | 1.7, 2.5, 3, 3.5, 5 | 2–10 | 17% | Yes | Yes | 60 / 60 °C | 130 | 185 | Hybrid silica, reversed-phase + anion exchange | Atlantis Premier BEH C18 AX |
| XP Oligo tC18 | L1 | 1.7, 2.5, 3, 3.5, 5 | 1–12 | 18% | Yes | Yes | 80 / 60 °C | 130 | 185 | Hybrid silica, C18, for oligonucleotides | XBridge Oligonucleotide BEH C18 |
* The USP class shown for XP NH2 is as published by the manufacturer and is being confirmed.
MicroPulite PHS XP
Surface-charged hybrid silica, 130 Å, designed for basic compounds in low-ionic-strength mobile phases.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| PHS XP tC18 | L1 | 1.7, 2.5, 3, 3.5, 5 | 1–11 | 15% | Yes | Yes | 80 / 45 °C | 130 | 185 | Surface-charged hybrid, C18 | XSelect CSH C18 |
| PHS XP F5 | L43 | 1.7, 2.5, 3, 3.5, 5 | 1–8 | 10% | No | No | 60 / 45 °C | 130 | 185 | Surface-charged hybrid, PFP | XSelect CSH Fluoro-Phenyl |
| PHS XP Phenyl-Hexyl | L11 | 1.7, 2.5, 3, 3.5, 5 | 1–11 | 14% | Yes | No | 80 / 45 °C | 130 | 185 | Surface-charged hybrid, phenyl-hexyl | XSelect CSH Phenyl-Hexyl |
MicroPulite Gold
High-purity silica, 120 Å, 300 m²/g. General-purpose reversed-phase and normal-phase columns for routine QC.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gold C18 | L1 | 3, 3.5, 5, 10 | 2–8 | 18% | Yes | No | 60 / 40 °C | 120 | 300 | Pure silica, C18 | Symmetry C18 |
| Gold C8 | L7 | 3, 3.5, 5 | 2–8 | 12% | Yes | No | 60 / 40 °C | 120 | 300 | Pure silica, C8 | Symmetry C8 |
| Gold C4 | L26 | 3, 3.5, 5 | 2–8 | 3.9% | Yes | No | 60 / 40 °C | 120 | 300 | Pure silica, C4 | — |
| Gold RP18 | L1 | 3, 3.5, 5 | 1–8 | 20% | Yes | Yes | 60 / 40 °C | 120 | 300 | Pure silica, amide C18 | — |
| Gold RP18 Plus | L1 | 3, 3.5, 5 | 2–8 | 17% | Yes | Yes | 60 / 40 °C | 120 | 300 | Pure silica, carbamate C18 | Symmetry Shield RP18 |
| Gold RP8 Plus | L7 | 3, 3.5, 5 | 2–8 | 15% | Yes | Yes | 60 / 40 °C | 120 | 300 | Pure silica, carbamate C8 | Symmetry Shield RP8 |
| Gold RP4 Plus | L26 | 3, 3.5, 5 | 2–8 | 3.9% | Yes | Yes | 60 / 40 °C | 120 | 300 | Pure silica, carbamate C4 | — |
| Gold Phenyl-Hexyl | L11 | 3, 3.5, 5 | 2–8 | 13% | Yes | No | 60 / 40 °C | 120 | 300 | Pure silica, phenyl-hexyl | — |
| Gold PFP | L43 | 3, 3.5, 5 | 2–8 | 10% | Yes | No | 60 / 40 °C | 120 | 300 | Pure silica, pentafluorophenyl | — |
| Gold CN | L10 | 5 | 2–8 | 5% | Yes | No | 60 / 40 °C | 120 | 300 | Pure silica, cyano | — |
| Gold NH2 | L8 | 3, 3.5, 5 | 2–8 | 3.8% | No | No | 60 / 40 °C | 120 | 300 | Pure silica, amino | — |
| Gold HILIC | L3 | 3, 3.5, 5, 10 | 2–7 | — | No | No | 60 / 40 °C | 120 | 300 | Pure silica, bare | — |
MicroPulite Platinum
High-purity silica, 100 Å, 320 m²/g. High surface area for strong retention.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Platinum C18 | L1 | 3, 3.5, 5 | 1–8 | 16% | Yes | No | 50 / 40 °C | 100 | 320 | Pure silica, C18, bifunctional | SunFire C18 |
| Platinum C8 | L7 | 3.5, 5 | 1–8 | 12% | Yes | No | 40 / 40 °C | 100 | 320 | Pure silica, C8, bifunctional | SunFire C8 |
MicroPulite Perfect
Pure silica, 120 Å. Low-density C18 for polar compounds in fully aqueous mobile phases, diol and HILIC.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Perfect T3 | L1 | 3, 5 | 1–8 | 14% | Yes | Yes | 45 °C | 120 | 300 | Pure silica, low-density C18 | Atlantis T3 |
| Perfect T3 SB | L1 | 3, 5 | 1–8 | 14% | No | Yes | 45 °C | 120 | 300 | Pure silica, low-density C18, not end-capped | — |
| Perfect Diol | L20 | 3, 5 | 1–8 | 15% | Yes | No | 60 °C | 120 | 300 | Pure silica, diol | — |
| Perfect HILIC | L3 | 3, 5 | 1–5 | — | No | No | 45 °C | 120 | 300 | Pure silica, bare | Atlantis HILIC Silica |
MicroPulite HSS
High-strength silica, 100 Å. Low-density C18 for UHPLC pressures and polar retention.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| HSS T3 | L1 | 1.8, 2.5, 3.5 | 1–8 | 11% | Yes | Yes | 60 / 40 °C | 100 | 230 | High-strength silica, low-density C18 | XSelect HSS T3 |
BioPulite
Wide-pore (300 Å) phases for peptides, proteins, monoclonal antibodies and oligonucleotides, and PEG-bonded size-exclusion phases at 125 Å and 300 Å.
| Phase | USP class | Particle sizes (µm) | pH | Carbon load | End-capped | 100% aqueous | Max. temp. (low / high pH) | Pore (Å) | Surface area (m²/g) | Bonding | Comparable phase ¹ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| BioPulite XP Protein tC18 | L1 | 1.7, 2.5, 3.5, 5 | 1–12 | 12% | Yes | Yes | 80 / 60 °C | 300 | 100 | Hybrid silica, C18, wide pore | XBridge Peptide BEH C18 |
| BioPulite XP Protein tC4 | L26 | 1.7, 2.5, 3.5, 5 | 1–12 | 8% | Yes | Yes | 80 / 50 °C | 300 | 100 | Hybrid silica, C4, wide pore | XBridge Protein BEH C4 |
| BioPulite XP Protein RP18 | L1 | 3.5, 5 | 2–11 | 12% | Yes | Yes | 50 / 45 °C | 300 | 100 | Hybrid silica, amide C18, wide pore | — |
| BioPulite XP Protein RP18 Plus | L1 | 3.5, 5 | 2–11 | 17% | Yes | Yes | 50 / 45 °C | 300 | 100 | Hybrid silica, carbamate C18, wide pore | — |
| BioPulite Gold Protein C18 | L1 | 3, 5 | 2–8 | 8.5% | Yes | No | 60 / 40 °C | 300 | 100 | Pure silica, C18, wide pore | Symmetry300 C18 |
| BioPulite Gold Protein C4 | L26 | 3, 5 | 2–8 | 1.3% | Yes | No | 60 / 40 °C | 300 | 100 | Pure silica, C4, wide pore | Symmetry300 C4 |
| BioPulite Perfect SEC 125 | L33 | 3, 5 | 2–8 | — | No | Yes | 45 °C | 125 | 300 | Pure silica, PEG-bonded, size exclusion | — |
| BioPulite Perfect SEC 300 | L33 | 5 | 2–8 | — | No | Yes | 45 °C | 300 | 100 | Pure silica, PEG-bonded, size exclusion | — |
PrePulite
Preparative columns and bulk packing media in the same chemistries as the analytical phases, for direct scale-up.
WeChromlite guard system
A reusable guard holder takes replaceable guard cartridges matched to each analytical chemistry. Replace the cartridge, keep the holder, and protect the analytical column.
| Holder | For columns of internal diameter | Type |
|---|---|---|
| GC-21 | 2.1 mm | Direct-connect, analytical |
| GC-30 | 3.0 mm | Direct-connect, analytical |
| GC-46 | 4.6 mm | Direct-connect, analytical |
| GC-100 | 10 mm | Stand-alone, semi-preparative |
| GC-190 / GC-200 | 20 – 30 mm | Stand-alone, semi-preparative |
| GC-300 | 30 – 50 mm | Stand-alone, semi-preparative |
Cartridges are sold in packs of three, in the same phase and particle size as the analytical column.
Documents available for every product
- Certificate of Analysis, issued for every batch and traceable by lot number
- Technical data sheet and safety data sheet
- Product range and USP L-classification cross-reference (all phases)
- ISO 9001:2015 certificate, FDA DMF summary, certificate of origin
- Supplier qualification package for quality units (eight documents)
¹ Comparable phase: a column of the same USP classification and similar chemistry, given for identification only. XBridge, XSelect, Symmetry, SunFire and Atlantis are trademarks of Waters Corporation, which is not affiliated with Cool Earth or WePure. Where a validated or compendial method is in use, how a change of column is verified remains a matter for the laboratory's own quality system. Temperatures: maximum at low pH / at high pH.